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- ArticleSawyer HW, Dabscheck R, Nott PR, Selinger BK, Kuntz ID.Biochem J. 1975 Jun;147(3):613-5.The hydrodynamic changes which accompany the dissociation of metal ions, from concanavalin A at acid pH are a result of charge effects rather than of dissociation of metal ions as such. Measurements of the rotational relaxation time are discussed in terms of the hydration of the protein and its polymeric heterogeneity.